منابع مشابه
Specific binding of thionine to the active site of trypsin.
The binding of thionine (3,6-diaminophenothiazine) to trypsin is associated with a red shift in the visible absorption spectrum of the dye. Spectrophotometric titration and equilibrium dialysis measurements are consistent with the presence of a single thionine-binding site on the trypsin molecule. At pH 7 and 4O, &is8 for the trypsin-thionine complex is 1.2 X lOUs M. The dye is displaced from t...
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A sensitive immunoradiometric assay, without an enzymatic step and specific for active human renin, was developed with use of two monoclonal antibodies (MAbs). In this assay system, the first MAb was coupled to magnetic beads (Magnogel); the second one, directed against the active form of the enzyme, was radiolabeled with 125I. The specificity of this assay was demonstrated in experiments measu...
متن کاملIncidence and specificity of interference in two-site immunoassays.
Using a modified "two-site" immunoradiometric assay, termed an "interference assay," we have demonstrated the occurrence of non-analyte antibody-binding substances in approximately 40% of serum samples. These substances multivalently bind antibodies from any of several species at a site other than the antigen-binding site. Using a two-site immunoradiometric assay for human choriogonadotropin, w...
متن کاملInterference by human anti-mouse antibody in two-site immunoassays.
Studies with goat and rabbit anti-mouse antibody, as models of human anti-mouse antibody (HAMA), have shown that several of the commonly used two -site immunoassays (e.g., for CA-125, carcinoembryonic antigen, choriogonadotropin, lutropin, hepatitis B surface antigen, thyrotropin) are susceptible to interference by this type of bridging heterophile antibody. In most cases the interference can b...
متن کاملUtilization of Site-Specific Recombination in Biopharmaceutical Production
Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...
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ژورنال
عنوان ژورنال: Blood
سال: 1990
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v76.4.755.bloodjournal764755